Genetically Modified Plants And Animals Biology Essay

Genetically Modified Plants And Animals Biology Essay The genome of both animals and plants has been altered for many years through various traditional breeding methods and organisms with favourable characteristics have being artificially selected to breed successive generations. These methods, however, have been limited to naturally occurring variations. Recent advances in genetic engineering have made it possible for scientists to precisely control any genetic changes introduced into an organism. Genes from one species can, through genetic engineering, be incorporated into an entirely different species. Any organism which has had its genetic material altered is defined as a genetically modified organism (GMO). The definition states that a GMO includes any living organism that possesses a novel combination of genetic material obtained through the use of modern biotechnology. It is important to note that in the case of humans, even if they have had their genes altered as a result of, gene therapy for instance, they are not considered to be GMOs. A major purpose of the field of genetics is to classify genes according to their function. The scientific study of genes in living organisms can be separated into three different strategies. The first two, which analyse natural variation and random mutagenesis, are the primary methods of forward genetics where the genotype of an abnormal phenotype is studied. In the case of random mutagenesis, it is often difficult to trace a phenotype back to a specific gene as many chromosomal loci are simultaneously targeted. Thus a third type of research strategy, reverse genetic techniques, has been developed in the past three decades. This method, where a specific gene is modified and the phenotype is subsequently investigated, provided tools for the research of gene function in a targeted manner. Among the most frequently used animals are Drosophila melanogaster (fruit fly), Caenorhabditis elegans (nematode) and Mus musculus (house mouse), and plants Nicotiana tabacum (tobacco), Arabidopsis thaliana (thale cress) and Triticum aestivum (common wheat) which have each been essential for the identification of genes implicated in aging, cell differentiation, development, and other significant biological functions. Transgenic rats are of great importance in neuroscience as they have been extensively used in behavioural paradigms (Abbott 2004) while recently, the first transgenic primate disease model, for Huntingtons disease, was created (Yang et al. 2008). Research has progressed through the use of various techniques. One of these techniques is the loss of function method where an organism is modified so that one or more genes lose their activity. This method has allowed researchers to analyze defects caused by particular mutations and has been extremely useful in identifying the function of a gene. Another method is the knockout experiment which involves the creation and manipulation of a DNA construct in vitro. In a simple knockout, this construct contains a copy of the required gene which has been slightly changed to lose its function. The modified gene is then taken up by embryonic stem cells, and it replaces the organisms own gene. These stem cells are then injected into a blastocyst which is implanted into a surrogate mother. Furthermore, the gain of function method is frequently performed in conjunction with the knockout method so that the function of a desired gene can be more finely identified. The process is very similar to knockout engineering, but in this case the construct amplifies the function of the gene, by adding extra copies of the gene or inducing more frequent synthesis of the protein. Information on the localization and interaction of a protein can be obtained through tracking experiments. In this method the wild-type gene is replaced with a fusion gene, which is a combination of the wild-type gene with a reporting element such as Green Fluorescent Protein (GFP). The reporting element allows visualization of the products of the modification. Moreover, expression studies can display the time and location of the synthesis of the protein. In this technique the promoter is reintroduced into an organism but the coding region is replaced by a reporting element or an enzyme catalyzing the production of a dye. A further advancement in expression studies has been the process promoter bashing, where the promoter is altered so as to find which pieces are crucial for proper gene expression and are bound by transcription factor proteins. The use of these, and other, methods to investigate critical questions in genetics has become standard practice. In particular, GMOs have been essential for 1) Identifying a genes function and any molecular elements related to it; 2) Creating models of human diseases; 3) Determining and confirming drug targets and specificity and 4) Investigating chronological aspects of gene function. 1) Identifying a genes function and any molecular elements related to it Targeted mutagenesis has been used extensively in the field of neuroscience. One of the very first groundbreaking experiments using targeted mutagenesis was carried out by Eric Kandel at Columbia University. Using the tetracycline inducible system, they expressed a calcium-independent form of the calcium dependent kinase, calcium-calmodulin kinase II (CaMKII), which is specific for the forebrain. They also observed a decline in spatial memory and hippocampal long-term potentiation (LTP). This major breakthrough displayed how both temporal and spatial control over molecular elements can help to identify the function of a particular gene and its role in brain function. Plant modification techniques have been invaluable tools for genetic research. Through the use of these methods, geneticists have gained detailed knowledge on the function of specific plant genes, their expression and the properties of the proteins they encode. One important trait that has been studied using transgenic plants is plant mechanical strength, which has an agronomic significance. Transgenic rice from the Indica variety was used to elucidate the molecular mechanism controlling the mechanical strength of crops. Mutations on the BC1 gene, which is expressed mostly in developing sclerenchyma cells and vascular bundles, were introduced into rice and resulted in a decrease of cell wall thickness and cellulose content and an increase in lignin level. This suggested that BC1 has a vital role in the biosynthesis of the cell walls of mechanical tissues. 2) Creating models of human diseases Another significant use for GMOs is the development of models for human disorders. This can be done by introducing a mutated gene or eliminating a gene which has a putative role in the disease. GMOs have been tremendously useful in modelling a wide range of illnesses, especially in the field of neuroscience. These include Alzheimers disease (AD), Huntingtons disease, cerebral ischemia and neuropsychiatric disorders. AD, for instance, is characterized by the presence of neurofibrillary tangles formed by hyper-phosphorylated tau protein and by amyloid ÃŽÂ ²-peptide (AÃŽÂ ²) plaques. Mutations in the amyloid precursor protein (APP), apolipoprotein E (APOE) and presenilin 1 and 2 (PS1, PS2), have all been shown to have a role in the disease. Research on transgenic mice has indicated that an overexpression of APP and PS1 causes AÃŽÂ ² plaque formation and leads to memory deficits, which are both symptoms of AD. The significant role of GMOs in testing potential causal mechanisms o f human disease is consequently highlighted. Recently, researchers at The University of Western Ontario working with scientists in Brazil used a unique transgenic mouse line to discover a previously unknown mechanism causing heart failure. The study showed that if the release of the neurotransmitter acetylcholine, which decreases cardiac activity, is reduced the probability of heart failure increases (ScienceDaily, 2010). Fundamental discoveries made first in plants have been central in our understanding of human biology, specifically cells, genes, molecular chaperones, transposable elements, programmed cell death, and gaseous hormones. Research on  genetically modified A. thaliana has helped in the identification of genes involved in human disease.  Scientists have shown that eighty-eight genes on chromosome 5 of A. thaliana are very similar to the 289 genes linked to human disease syndromes which have been established for comparison with D. melanogaster. As the majority of these are also greatly conserved between D. melanogaster and C. elegans, A. thaliana biology can be modified and used as a model to increase our knowledge of human disease. Many of these genes encode proteins which have a conserved function. For example, DNA excision repair genes (linked to xeroderma pigmentosum) and ATP-dependent copper transporters (linked to Wilsons and Menkes disease). In the latter the A. thaliana homolog ue is more similar to the human homologue than to the D. melanogaster or C. elegans counterparts. 3) Determining and confirming drug targets and specificity The development of genetic models for diseases has greatly assisted in drug discovery and in identifying drug targets. Genetic modification can be extremely specific, for instance removing a gene which codes for a particular receptor subtype. This specificity ensures almost complete selectivity and is thus preferred to classical pharmacological approaches. For example, through the use of both the knock-in and knockout methods scientists can examine the function of receptor subtypes. Specifically, transgenic mice have been used to establish the purpose of specific GABA receptor subunits in the diverse actions of diazepam. Diazepam, a benzodiazepine, acts on GABAA receptors which consist of ÃŽÂ ±1-, ÃŽÂ ±2-, ÃŽÂ ±3-, or ÃŽÂ ±5- subunits. The mice where modified to carry point mutations in the benzodiazepine receptors of each of their subunits, and the investigators were thus able to genetically separate the distinct functions of diazepam (e.g. sedative or anxiolytic) acting at otherwise similar GABAA receptors. Plants are very significant as novel therapeutic drug leads. Nicotine, the main biologically active compound in N. tabacum, binds to the nicotinic acetylcholine receptors (nicAChRs) in the central nervous system (CNS) to produce a wide variety of biological effects. The nicAChR family consists of receptors made of ÃŽÂ ²-subunits and a series of structurally varied ÃŽÂ ±-subunits. The differences in the ÃŽÂ ±-subunit amino acids and in the subunit composition create variations in the receptors binding site and give the members of this receptor family unique drug specificity. Through the use of transgenic N. tabacum it was demonstrated that a significant number of the plants contained compounds that are selective for brain receptors linked to the a7 nicAChR. Compounds which have this selectivity are of particular interest as drugs that act on these receptors may be useful to treat degenerative brain diseases such as AD. 4) Determining and confirming drug targets and specificity In addition, GMOs have been used to analyse second messenger signaling pathways and also to determine vital developmental timing of gene function. The latter was demonstrated by using a tetracycline inducible knock-out of the serotonin 1A receptor. When the receptor was knocked out during development, it caused the knockout mouse to experience increased anxiety. When the receptor was knocked out in adults, however, the anxiety levels were normal. It was thus shown that the 1A receptor is associated to a developmental factor necessary for normal emotional behaviour. Transgenic plants have also been effectively used to analyze regulated gene expression, as the expression can be investigated at various stages and in different tissues. The environmental factors that influence gene expression and that have been studied in transgenic plants include temperature, light intensity, anaerobic stress, and wounding. The development of flowers requires the collaboration of specialized tissues. Research was carried out on transgenic petunia to determine the genes controlling the development and differentiation in the flower. By analysing the 5-enolpyruvylshikimate-3-phosphate synthase gene, which is found in high concentrations in flowers, the researchers were able to identify an upstream region that is responsible for the tissue-specific and developmentally regulated expression. Conclusion In summary, this technology has played a considerable role in genetics. The most common applications of GMOs in the field of genetics have been highlighted: dissecting biological mechanisms, modelling human diseases, discovering and validating pharmaceuticals, and investigating crucial time windows in gene function. While there are many other types of approaches for creating GMOs and many other uses for these organisms, the role of GMOs in genetics has being the main focus, as the impact on this field has been substantial. New approaches to creating and using GMOs are continuously being developed, including adjustments and combinations of the discussed techniques, which will surely further impact genetics.

Communication in Marriage Essay

Marriage is a beautiful thing, but at times can be very challenging. Making a commitment and keeping it takes a lot of work and patience. One reason that a marriage may face a problem is due to the lack of communication. At certain times women may need someone to hear them out whereas on the other hand men want someone to give them advice and support. In â€Å"His Talk, Her Talk† by Joyce Maynard, the author introduces us to her marriage and communication between her and her husband. In the other article â€Å"Man to Man, Women to Women† by Mark A. Sherman and Adelaide Haas, talks more about how men talk more directly and women think, and watch what they say. In â€Å"His Talk, Her Talk† by Joyce Maynard, she gives us an outlook on her marriage and the way she communicates with her husband. Maynard states that the opposite sexes are usually excluded from the conversation, such as the idea that there is a thing as â€Å"men’s talk† or â€Å"women’s talk† (Maynard 31). Men and women usually divide into two groups when there is a party or any sort of gathering. Immediately we redistributed ourselves which was a shame (Maynard 31). This tends to happen often and one may wonder as to why? Why can’t men and women have a long conversation about certain subject that both are interested in? Some women may argue that speaking to another woman, their point will be taken a lot more seriously whereas men tend to disregard the entire point altogether. Men like to have more simple straight forward conversation that gets to the point. Women are more verbal and they like to express themselves and detail their conv ersations. In the article â€Å"Man to Man, Women to Woman† by Mark A. Sherman and Adelaide Haas, the authors use a questionnaires, interviews, and observations to see how different types of genders communicate. Herman and Haas, in their research tested one hundred sixty-six women and 110 men, ranging in age from seventeen to 80, returned a questionnaire asking how often they discussed each of 22 topics with friends of the same sex (Herman and Haas 33). Women’s topic tends to be closer to the self and more emotional than men’s (Sherman and Haas 33). This does not mean that men do not have emotional talks; it just takes a right person to bring it out of them. As stated in the article men can be good listeners, as well as women can give direct advice (Sherman and Haas 33). Communication is key to any happy relationship whether it may be a marriage, girlfriends or just male companionship, women and men due indeed complete each other. When a woman is full of emotions, the man is always there to comfort her. Men tend to bottle up their emotions and they know that they can open up to a woman for emotional support. The first article â€Å"His Talk, Her Talk† by Joyce Maynard, was acknowledgement of her own personal life, whereas the second article â€Å"Man to Man, Woman to Woman† by Sherman and Haas, was based on the questionnaire and observation of other women and men and how they communicate. I would recommend the article â€Å"Man to Man, Women to Women† by Sherman and Haas, because it is well written, explaining the core problems that men and women have in their communication between one another. Citation Maynard, Joyce, †His Talk, Her Talk† Text and Contexts: A Contemporary Approach to College Writing. Ed. William S. Robinson et al. Boston Wadsworth, 2009. Print Sherman, Mark and Haas, Adelaide. â€Å" Man to Man, Woman to Woman† Text and Contexts: A Contemporary Approach to College Writing. Ed. William S. Robinson et al. Boston Wadsworth, 2009. Print

Michael Graves Essay

Art Appreciation Michael Graves Michael Graves is a post-modern architect born in Indianapolis, Indiana on July 9th 1934. He graduated from Broad Ripple High School; he then attended and got his bachelor degree at the University of Cincinnati. After getting his bachelors, he went to Harvard where he got his masters in Interior design. After college he started career as an architect, it was in 1968 when he designed and built the Hanselmann House in Fort Wayne, Indiana. He built houses for about 10 years until he started building all kinds of buildings. He built anything from the Dolphin Resort at Disney world from the Denver Public Library. Graves has been designing buildings of all types since 1968 very rapidly, some years designing four or more buildings a year. One of Graves’s most famous buildings is the Portland building in Oregon. This building was made in 1980 and is unique because of how he surprised everyone with the exterior design. He used a pair of classical columns that have no structural function. He made these columns stand out by setting them in a reflecting pool. The Portland public says the building is both formal and playful, governor Ivancie says that most big city building were beginning to be built to simple with Just glass outsides. He says the Portland building doesn’t lack design features like other big city buildings. Some did criticize the design but most were thrilled by the uniqueness of the new building. People say there is type of humor to his designs and buildings. â€Å"Graves has become an opponent of modern works who uses humor as an integral part of his architecture† (1). Graves uses designs from the past and puts his own twist on them. â€Å"Graves re-interpreted the rational style that had been introduced by Le Corbusier in the 1920s into a neoclassical style† (1). Michael Graves has many building that are very famous, but his design on the Humana Building in Louisville, Kentucky is maybe his best design he’s had. His design on this building got him in the time magazine. â€Å"TIME Magazine listed it as one of the 10 best buildings of the decade† (2). The building looks different from every side. Many say it is one of the most unique and distinctive skyscrapers in America. Critics say it is a textbook example of Postmodernism. Grave’s Humana building is said to have its fair share of flaws but the brilliant design more than counteracts the flaws. â€Å"While the building has undeniable flaws, they are easily outweighed by its attributes, hich have only grown stronger with time†(3). http://featuresblogs.chicagotribune.com/entertainment_tv/house/ http://www.chicagotribune.com/entertainment/tv/ http://featuresblogs.chicagotribune.com/technology_internetcritic/ https://books.google.com.ua/books?id=JwhZCwAAQBAJ&pg=PA225&lpg=PA225&dq=http://featuresblogs.+chicagotribune.+com/t&source=bl&ots=CwG3pAKd5l&sig=5KrPfyo2wN90yu3ZXrsblMPOpew&hl=uk&sa=X&ved=0ahUKEwjy0NvQhoPUAhVKD5oKHUzSCe8Q6AEIRDAE#v=onepage&q=http%3A%2F%2Ffeaturesblogs.%20chicagotribune.%20com%2Ft&f=false https://en.wikipedia.org/wiki/Humana_Building Humana Humana - Michael Graves Architecture & Design window._wpemojiSettings = {"baseUrl":"https:\/\/s.w.org\/images\/core\/emoji\/11\/72x72\/","ext":".png","svgUrl":"https:\/\/s.w.org\/images\/core\/emoji\/11\/svg\/","svgExt":".svg","source":{"concatemoji":"https:\/\/www.michaelgraves.com\/wp-includes\/js\/wp-emoji-release.min.js?ver=4.9.8"}}; !function(a,b,c){function d(a,b){var c=String.fromCharCode;l.clearRect(0,0,k.width,k.height),l.fillText(c.apply(this,a),0,0);var d=k.toDataURL();l.clearRect(0,0,k.width,k.height),l.fillText(c.apply(this,b),0,0);var e=k.toDataURL();return d===e}function e(a){var b;if(!l||!l.fillText)return!1;switch(l.textBaseline="top",l.font="600 32px Arial",a){case"flag":return!(b=d([55356,56826,55356,56819],[55356,56826,8203,55356,56819]))&&(b=d([55356,57332,56128,56423,56128,56418,56128,56421,56128,56430,56128,56423,56128,56447],[55356,57332,8203,56128,56423,8203,56128,56418,8203,56128,56421,8203,56128,56430,8203,56128,56423,8203,56128,56447]),!b);case"emoji":return b=d([55358,56760,9792,65039],[55358,56760,8203,9792,65039]),!b}return!1}function f(a){var c=b.createElement("script");c.src=a,c.defer=c.type="text/javascript",b.getElementsByTagName("head")[0].appendChild(c)}var g,h,i,j,k=b.createElement("canvas"),l=k.getContext&&k.getContext("2d");for(j=Array("flag","emoji"),c.supports={everything: !0,everythingExceptFlag:!0},i=0;i

Bin Ladens 1996 Declaration of War on the U.S.

On August 23, 1996, Osama bin Laden signed and issued the Declaration of Jihad Against the Americans Occupying the Land of the Two Holy Mosques, meaning Saudi Arabia. It was the first of two explicit declarations of war against the United States. The declaration summed up bin Ladens belief, categorical and uncompromising, that there is nothing more imperative, after faith, than to repel the aggressor who corrupts religion and life, unconditionally, as far as possible. In that line was the seed of bin Ladens stance that even the killing of innocent civilians was justified in defense of the faith. American forces were encamped in Saudi Arabia since 1990 when Operation Desert Shield became the first step in the war to oust Saddam Husseins army from Kuwait. Abiding by extreme interpretations of Islam that the overwhelming majority of Muslim clerics around the world reject, bin Laden considered the presence of foreign troops on Saudi soil an affront to Islam. He had, in 1990, approached the Saudi government and offered to organize his own campaign to oust Saddam Hussein from Kuwait. The government politely rebuffed the offer. Until 1996, bin Laden, at least in the Western press, was an obscure figure occasionally referred to as a Saudi financier and militant. He was blamed for two bombings in Saudi Arabia in the previous eight months, including a bombing in Dhahran that killed 19 Americans. Bin Laden denied involvement. He was also known as one of the sons of Mohammed bin Laden, the developer and founder of the Bin laden Group and one of the richest men in Saudi Arabia outside the royal family. The bin Laden Group is still Saudi Arabias leading construction firm. By 1996, bin laden had been expelled from Saudi Arabia, his Saudi passport having been revoked in 1994, and expelled from Sudan, where he had established terrorist training camps and various legitimate businesses. He was welcomed by the Taliban in Afghanistan, but not exclusively out of the goodness of Mullah Omar, the Taliban leader. To maintain good graces with the Taliban, Steve Coll writes in The bin Ladens, a history of the bin Laden clan (V iking Press, 2008), Osama had to raise about $20 million per year for training camps, weapons, salaries, and subsidies for the families of volunteers. [...] Some of these budgets overlapped with business and construction projects Osama engaged in to please Mullah Omar. Yet bin Laden felt isolated in Afghanistan, marginalized and irrelevant. The declaration of jihad was the first of two explicit declarations of war against the United States. Fund-raising may very well have been part of the motive: by raising his profile, bin Laden was also drawing more interest from the sympathetic charities and individuals underwriting his efforts in Afghanistan. The second declaration of war was to be delivered in February 1998 and would include the West and Israel, giving certain donors even more incentive to contribute to the cause. By declaring war on the United States from a cave in Afghanistan, wrote Lawrence Wright in The Looming Tower, bin Laden assumed the role of an uncorrupted, indomitable primitive standing against the awesome power of the secular, scientific, technological Goliath; he was fighting modernity itself. It did not matter that bin Laden, the construction magnate, had built the cave using heavy machinery and that he had proceeded to outfit it with computers and advanced communications devices. The stance of the primitive was appealingly potent, especially to people who had been let down by modernity; however, the mind that understood such symbolism, and how it could be manipulated, was sophisticated and modern in the extreme. Bin Laden issued the 1996 declaration from the southern mountains of Afghanistan. It appeared on Aug. 31 in al Quds, a newspaper published in London. The response from the Clinton administration was close to indifferent. American forces in Saudi Arabia had been on a higher state of alert since the bombings, but bin Ladens threats changed nothing. Read the Text of bin Ladens 1996 Jihad Declaration